Abstract : The vinegar produced from locally grown pineapple and pawpaw (with and without their peels) were evaluated to determine their antimicrobial properties on some clinical isolates. Agar well diffusion method was used for this analysis. The zones of inhibitions were measured in millimeters.  The results of the antimicrobial analysis showed that the vinegar exhibit antimicrobial activities on the clinical isolates.On Escherichia colithere was  8 ± 1.5mm diameters for the aqueous extract of the pineapple juice vinegar and 11 ± 0.44mm for the methanol extract of the pineapple peel vinegar, 10 ±1.23mm and 12 ±0.19 for the aqueous and methanol extract of  pawpaw juice vinegar and 6 ±0.36mm and 7 ±0.77mm for the aqueous and methanol extract of  pawpaw peel vinegar respectively. On Staphylococcus aureus, there was 9 ± 0.50mm and 10 ±1.00mm for the aqueous and methanol extract of the pineapple juice vinegar respectively while the pineapple peel vinegar extract showed 10 ± 1.50mm and 7 ±0.50mm for the aqueous and methanol extract, 7 ±0.29mm and 8 ±0.77 for aqueous and the methanol extract of the pawpaw juice vinegar, 10±0.55mm and 7 ±0.86mm for aqueous and methanol extract of pawpaw peel vinegar. On Bacillus sp there was  9 ± 0.99mm diameter for the aqueous extract of the pineapple juice vinegar and 8 ±1.30mm for the methanol extract,  9 ±0.98mm  and 7 ± 0.99mm for the aqueous and  the methanol extract of the pineapple peel vinegar, 9 ±0.55mm aqueousextract of  pawpaw juice vinegar and 13±0.76mm for aqueous extract of pineapple peel vinegar. On Candida albicans there 8 ± 0.12mm diameter for the aqueous extract of the pineapple juice vinegar and 9 ± 0.99mm for the methanol extract10 ±1.00mm   and 7 ± 0.76mm for the aqueous and the methanol extract of the pineapple peel vinegar, 8 ±0.65mm and 7 ±0.9mm for aqueous and methanol extract of pawpaw juice vinegar and 9±1.22mm and 10 ±0.22mm for aqueous and methanol extract of pineapple peel vinegar. This shows that the produced vinegars exhibited antimicrobial characteristics and their use should be encouraged .